Mass spectrometry (often “mass spec” or MS) is the standard way to confirm a peptide’s identity. While HPLC measures how pure a sample is, mass spec answers a different question: is this actually the right molecule? Here’s a plain-English overview.
- What it does: measures a molecule’s mass to confirm its identity.
- Pairs with HPLC: purity (HPLC) + identity (MS) together.
- On the COA: a good Certificate of Analysis reports both.
How it works
A mass spectrometer ionizes the molecules in a sample and measures their mass-to-charge ratio. The result is a precise molecular weight. If that measured weight matches the expected weight for the peptide’s sequence, the identity is confirmed — see peptide molecular weight.
Purity vs identity
| Question | Method |
|---|---|
| Is it the right molecule? | Mass spectrometry |
| How much is the target? | HPLC |
| Where are both reported? | Certificate of Analysis |
Both tests together give a complete picture of what’s in a vial.
Research use only. For educational purposes. Compounds referenced are sold strictly for in vitro laboratory research by qualified professionals.
Every batch we sell is identity- & purity-verified
Third-party Certificates of Analysis on each lot.