Disulfide Bonds in Peptides
Disulfide bonds in peptides: the cysteine 'bridges' that lock a peptide into a stable shape, and how they differ from peptide bonds.
Lab & Handling
Net Peptide Content vs HPLC Purity
Net peptide content vs HPLC purity: two different numbers on a spec sheet, and what each tells you about how much peptide is in the vial.
Sterile Filtration of Peptides
Sterile filtration of peptides: how a 0.22 micron filter removes microbes and particles, and what it does and doesn't remove.
TFA vs Acetate: Peptide Salt Forms
TFA vs acetate peptide salts: why research peptides come as salts, how the two forms differ, and when a TFA-free form is preferred.
Peptide Aggregation Explained
Peptide aggregation: why peptide molecules clump together, the warning signs, and how to reduce the risk in the lab.
What Is a Stock Solution?
What is a stock solution? A concentrated master solution you dilute as needed — why it keeps research consistent and accurate.
Aliquoting Peptides: Why & How
Aliquoting peptides: why splitting a solution into single-use portions protects your material and avoids repeated freeze-thaw cycles.
What Is Mass Spectrometry?
What is mass spectrometry? How it confirms a peptide's identity by measuring molecular weight, and how it pairs with HPLC purity testing.
Peptide Solubility: What Dissolves Peptides
Why some peptides dissolve easily and others don't. A simple guide to peptide solubility, diluents, and getting a clear solution.