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Peptide Basics

The 20 Amino Acids: A Quick Reference

The 20 amino acids quick reference featured image

Every peptide and protein is built from the same set of 20 standard amino acids. Their order determines what a peptide does. Here’s a quick reference to the building blocks.

  • 20 standard amino acids make up nearly all peptides and proteins.
  • Each has a three-letter and one-letter code.
  • They differ by their side chain, which gives each one its character.

The building blocks

All 20 share the same backbone — an amine group, a central carbon, and an acid group — and differ only in their side chain. That side chain decides whether an amino acid is water-loving, water-fearing, charged, or neutral.

GroupExamples (1-letter)
Nonpolar / hydrophobicAla (A), Val (V), Leu (L), Ile (I), Phe (F)
Polar / unchargedSer (S), Thr (T), Asn (N), Gln (Q)
Positively chargedLys (K), Arg (R), His (H)
Negatively chargedAsp (D), Glu (E)
Special casesGly (G), Pro (P), Cys (C)

Why the order matters

The same 20 letters can spell countless “words.” A peptide’s specific sequence — the order of its amino acids — gives it a unique shape and role. That’s why two peptides of the same length can behave completely differently.

Learn how sequences are written in Peptide Sequences & One-Letter Codes, or start with Peptides vs Amino Acids.

Research use only. For educational purposes. Compounds referenced are sold strictly for in vitro laboratory research by qualified professionals.

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